Med Bull Sisli Etfal Hosp. Accepted Articles: SETB-75301 | DOI: 10.14744/SEMB.2024.75301

The Antifibrotic Effects of Plasminogen Activator Inhibitor-1 Antagonists are Observed in Rats with Epidural Fibrosis

Pinar Kuru Bektasoglu¹, Adnan Somay², Julide Hazneci¹, Ali Borekci¹, Bora Gurer^3
1Department of Neurosurgery, University of Health Sciences Türkiye, Fatih Sultan Mehmet Training and Research Hospital, Istanbul, Türkiye
²Department of Pathology, University of Health Sciences Türkiye, Fatih Sultan Mehmet Training and Research Hospital, Istanbul, Türkiye
³Department of Neurosurgery, Istinye University Faculty of Medicine, Istanbul, Türkiye

Objective: Epidural fibrosis occurs after laminectomy as part of the local repair mechanisms. Adhesion around the nervous tissue could cause pain and disability. In the current study, we investigated the possible antifibrotic effects of the plasminogen activator inhibitor‑1 (PAI-1) antagonists in a rat laminectomy model.
Methods: The rats were randomly assigned to the control, the TM5441, and the TM5484 groups (n=6 per group). In the control group, just a laminectomy was performed. In the treatment groups, intragastric administration of PAI-1 antagonists was done after skin closure. Epidural fibrosis was investigated macroscopically and histopathologically four weeks later.
Results: In the TM5441 and TM5484 groups, the macroscopic epidural fibrosis score was less than the control group (p < 0.001 for both groups). Microscopic epidural fibrosis score was also decreased in the TM5441 and TM5484 groups (p > 0.05 for both groups). Fibroblast cell density classification scores in the TM5441 and TM5484 groups were lower when compared to the control group (p > 0.05 for both groups). Fibrosis thickness was lower in the TM5441 and TM5484 groups when compared to the control group (p < 0.01 for both groups).
Conclusions: Plasminogen activator inhibitor‑1 antagonists could be a treatment alternative for the prevention of epidural fibrosis.

Keywords: Epidural fibrosis, laminectomy, plasminogen activator inhibitor‑1 antagonists

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Plazminojen Aktivatör Ýnhibitör-1 Antagonistlerinin antifibrotik Etkileri Epidural Fibrozisli Sýçanlarda Gözlenmiþtir

Pinar Kuru Bektasoglu¹, Adnan Somay², Julide Hazneci¹, Ali Borekci¹, Bora Gurer^3
1Türkiye Saðlýk Bilimleri Üniversitesi, Fatih Sultan Mehmet Eðitim ve Araþtýrma Hastanesi, Beyin ve Sinir Cerrahisi Kliniði, Ýstanbul
²Türkiye Saðlýk Bilimleri Üniversitesi, Fatih Sultan Mehmet Eðitim ve Araþtýrma Hastanesi, Patoloji Kliniði, Ýstanbul
³Ýstinye Üniversitesi Týp Fakültesi, Beyin ve Sinir Cerrahisi Anabilim Dalý, Ýstanbul, Türkiye

Amaç: Epidural fibrozis, laminektomi sonrasý lokal onarým mekanizmalarýnýn bir parçasý olarak ortaya çýkar. Sinir dokusunun etrafýndaki yapýþma aðrýya ve sakatlýða neden olabilir. Bu çalýþmada, plazminojen aktivatör inhibitörü-1 (PAI-1) antagonistlerinin sýçan laminektomi modelinde olasý antifibrotik etkilerini araþtýrdýk.
Yöntemler: Sýçanlar rastgele olarak kontrol, TM5441 ve TM5484 gruplarýna (grup baþýna n=6) ayrýldý. Kontrol grubuna sadece laminektomi uygulandý. Tedavi gruplarýnda PAI-1 antagonistlerinin intragastrik uygulamasý cilt kapatýldýktan sonra yapýldý. Dört hafta sonra epidural fibrozis makroskobik ve histopatolojik olarak incelendi.
Bulgular: TM5441 ve TM5484 gruplarýnda makroskopik epidural fibrozis skoru kontrol grubuna göre daha düþüktü (her iki grup için p<0,001). Mikroskobik epidural fibrozis skoru da TM5441 ve TM5484 gruplarýnda azaldý (p>0,05, her iki grup için). TM5441 ve TM5484 gruplarýnda fibroblast hücre yoðunluðu sýnýflandýrma skorlarý kontrol grubuna göre daha düþüktü (her iki grup için de p>0,05). Fibrozis kalýnlýðý TM5441 ve TM5484 gruplarýnda kontrol grubuna göre daha düþüktü (her iki grup için p<0,01).
Sonuç: Plazminojen aktivatör inhibitörü‑1 antagonistleri epidural fibrozisin önlenmesinde bir tedavi alternatifi olabilir. (SETB-2024-11-180)

Anahtar Kelimeler: Epidural fibrozis, laminektomi, plazminojen aktivatör inhibitörü‑1 antagonistleri

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