Effects of the Folk Medicinal Plant Extract Ankaferd BloodStopper on the Healing of Colon Anastomosis: An Experimental Study in a Rat Model

Objectives: Ankaferd BloodStopper (ABS) is a topical hemostatic agent that modulates the inflammatory response and accelerates wound healing. The aim of this study was to determine the effects of ABS on the colon anastomosis wound healing in a rat model.
Methods: Thirty-two Wistar albino rats were divided into four groups as follows: Group A (n=8), left colonic anastomosis plus ABS treatment (sacrificed on the 3rd day); Group B (n=8) (control), left colonic anastomosis (sacrificed on the 3rd day); Group C (n=8), left colonic anastomosis plus ABS treatment (sacrificed on the 7th day); and Group D (n=8) (control), left colonic anastomosis (sacrificed on the 7th day). All rats were sacrificed at the end of the experiment to assess the anastomosis integrity and the presence of perianastomosis abscesses, peritonitis, and adhesions. Additionally, the bursting pressure and hydroxyproline (OH-pyroline) levels were determined, and a histopathologic evaluation of the perianastomosis tissue was conducted.
Results: The mean bursting pressure on Day 7 was significantly higher than that on Day 3 in the ABS group (p=0.017). Overall, the bursting pressure was higher in animals treated with ABS than in the control animals, although the difference was not statistically significant. The OH-pyroline levels of both ABS groups were significantly higher than in the control groups. The mean OH-pyroline level on Day 7 was higher than that on Day 3 in the ABS-treated animals (p=0.038).
Conclusion: ABS increases collagen formation and neovascularization, and it has a positive impact during the colon anastomosis healing in an experimental model of wound healing.

Geleneksel Bir Bitki Ekstresi, Ankaferd Kanama Durdurucunun Kolon Anastomozu Ýyileþme Süreci Üzerine Etkisi: Rat Modeli, Deneysel Bir Çaliþma

Amaç: Ankaferd Blood Stopper, enflamatuar yanýtý modüle eden ve yara iyileþmesini hýzlandýran topikal bir hemostatik ajandýr. Bu çalýþmanýn amacý, bir sýçan modelinde Ankaferd Blood Stopper'ýn kolon anastomozunun yara iyileþmesi üzerindeki etkilerini belirlemektir.
Gereç ve Yöntem: Otuz iki Wistar albino cinsi sýçan dört gruba ayrýldý: grup A (n = 8), sol kolonik anastomoz +ABS tedavisi (üçüncü gün sakrifiye); B grubu (n = 8) (kontrol), sol kolonik anastomoz (üçüncü gün sakrifiye); grup C (n = 8), sol kolonik anastomoz + ABS tedavisi ABS (yedinci gün sakrifiye); ve grup D (n = 8) (kontrol), sol kolonik anastomoz (yedinci gün sakrifiye). Anastomoz bütünlüðünü, perianatomotik apse varlýðýný, peritonit ve adezyon varlýðý deðerlendirildi. Ek olarak, patlama basýncý, hidroksiprolin (OH-pirolin) seviyeleri ve perianatomotik dokusunun histopatolojik özellikleri deðerlendirildi.
Bulgular: Genel olarak, patlama basýncý ABS ile tedavi edilen hayvanlarda kontrol hayvanlarýndan daha yüksekti, ancak fark istatistiksel olarak anlamlý deðildi. Her iki ABS grubunun OH-pirolin seviyeleri kontrol gruplarýndan anlamlý derecede yüksekti. Yedinci günde ortalama OH-pirolin seviyesi, ABS uygulanmýþ hayvanlarda üçüncü gündekinden daha yüksekti (p = 0.038).
Sonuçlar: ABS, deneysel yara iyileþmesi modelinde kollajen oluþumunu, neovaskülarizasyonu arttýrarak ve kolon anastomoz iyileþme süreci üzerinde olumlu bir etkiye sahiptir.