Med Bull Sisli Etfal Hosp. 2000; 34(1): 29-33

Measurement of Maternal Serum median HCG, afp and UE3 Hormon levels with triple screening test in Zeynep Kamil Hospital to predýct Prenatal risk

Ayþen Özkoral¹, Ýlter Güner¹, Seracettin Günaydýn¹, Bilge Türköver^2
1Zeynep Kamil Hastanesi, Biyokimya Laboratuvarý, Ýstanbul
²Zeynep Kamil Hastanesi, Genetik Laboratuvarý, Ýstanbul

OBJECTIVE: in the triple screening test performedfor the purpose of estimating the prenatal risk, tlýt differences displayed hy AFP, HCG and uE3 values in the serum as re/ated to the expected values for gestational week, were determined. in this study, our aim was to make it possible to attain pergect results, by way of estimating our own regional median values far pregnant women between the 14th and 20th gestational weeks who were admitted to the Hormone Laboratory at the Zeynep Kamil Hospita/ in order that the the median values of AFP, HCG and uE3; which were thought to bear regiona/ differences, were determined accurately.
STUDY DESIGN: This study was performed in 3012 pregnant women who were admitted to the Hormone Laboratory at the Zeynep Kamil Hospital between the 24th, October 1997 and the 27th, September 2000. The analyses of AFP, HCG and uE3 hormones in the maternal sera were carried aut.
RESULTS: The median values determined by way of MSAFP, MSHCG and MSuE3 measurement usedfor the purpose of estimating the prenatal risk in 3012 pregnant women, were found minimally different from the median values proposed by typo/og software,in 14, 15, 16, 17, 19th weeksfor MSHCG; in 14, 15, 16, 17, 18, 20th weeksfor MSAFP;in 14,20tn weeks far MsuE3. The prenatal risk,who were admitted admitted to the Genetic Laboratory at the Zeynep Kamil Hospital,an amniosynthesis was performed and two of them were diagnosed trisomic.
CONCLUSIONS: While a prenatal risk scre"e‘ning is being done,the median values of MSHCG,MSAFP and MSuE3 re/ated to the gestational week should be known on the basis of laboratory in which the test is practised.As the median values of these parameters are known to show social,even regional differences,it is a prerequisite that the median values displayed by the pregnant women admitted to our laboratory are estimated foran accurate practise of the test.

Keywords: Triple test,maternal serum maternal serum human chorionic gonadotropin(MSHCG),maternal serum free estrio(MSuE3)

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Prenatal Riskin Belirlenmesine Zeynep Kamil Hastanesinde Çalýþýlan Üçlü Tarama Testinde Maternal Serumdaki Hcg, Afp, Ue3 Hormonlarýnýn Median Degerlerinin Belirlenmesi

Ayþen Özkoral¹, Ýlter Güner¹, Seracettin Günaydýn¹, Bilge Türköver^2
1Zeynep Kamil Hastanesi, Biyokimya Laboratuvarý, Ýstanbul
²Zeynep Kamil Hastanesi, Genetik Laboratuvarý, Ýstanbul

AMAÇ: Prenatal riskin belirlenmesi amacýyla yapýlan üçlü tarama testinde,anne yaþýnýn getirdiði riske ilave olarak,maternal serumundaki AFP.HCG ve uEJ deðerlerinin gebelik haftalarýna göre olmasý gerekn deðerlerden farklýlýðý deðerlendirilir.Bu çalýþmada amacýmýz bölgesel farklýlýk/ar taþýdýðýna inanýlan MSHCG,MSAFP,MSHCG hormonlarýnýn median deðerlerinin doðru tespit edilmesi için Zeynep Kamil Hastanesi Hormon Laboratuvarýna baþvuran 14-20 gebelik haftasýndaki gebelerden kendi bölgesel median deðerlerimizi belirleyerek üçlü tarama testinde ideal sonuçlara ulaþmayý saðlamaktý.
MATERYAL VE METOD: Bu çalýþma 2411011997-27/9/2000 tarihleri arasýnda Zeynep Kamil Hastanesi Hormon Laboratuvarýna baþvuran 3012 gebe üzerinde yapýldý.Maternal serumda AFP,HCG,uE3 hormonlarýnýn analizi yapýldý.
BULGULAR: 3012 gebede prenatal risk belirleme amacýyla kul/andýðýmýz MSAFP,MSHCG,MSuE3 ölçümü ile elde ettiðimiz median deðerlerinin minimal de olsa typolog software‘ in önerdiði median deðerlerine göre MSHCG için 14, 15, 16, 17, /9.haftalarda,MSAFP için 14, 15, 16, 17, 18, 20‘inci haftalarda, MsuEJ için 14, 20.‘inci.haftalarda farklýlýklar gösterdiði tespit edildi. 3012 gebeden 149‘ unda prenatal risk bulundu. Prenatal risk tespit edilen gruptan Zeynep Kamil Hastanesi Genetik Laboratuvarýna baþvuran 28 gebenin amniosentez ile deðerlendirilmesi yapýldýðýnda 2 gebede trizomi tespit edildi.
SONUÇ: Prenata/ risk taramasý yapýlýrken.gebelik haftalarýna uyan MSHCG; MSAFP, MSuE3 medyan deðerlerinin testi uygulayan laboratuvar bazýnda bilinmesi gerekir.Bu parametrelerin medyan deðerlerinin toplumlar, hatta bölgesel fark/ ý l ýklar gösterebileceði düþünü/düð ünden, laboratuvarýmýza baþvuran gebelerin oluþturduðu medyan deðerlerin belirlenmesi, bu testin saðlýklý bir þekilde uygulanabilmesi iç_in bir önko_fuldur.

Anahtar Kelimeler: Uçlü Test,maternal serum alfafetoprotein(MSAFP), maternal serim human kornoyik gonatropin (MShCG), maternal serum serbest estriol(MSuEJ)

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